الفهرس 
Chapter I Introduction and aim of the workOnly 14 pages are availabe for public view
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Abstract
Both diabetes mellitus and varicocele are the most common diseases that present in many infertile patients. This study was conducted to overwhelm the negative response for the diabetes and varicocele semen by in vitro activation techniques using a medium containing herbal motility stimulants (i. e. Glycyrrhiza glabra). The medium used for in vitro activation was Hams F12 with 0.1% Glycyrrhiza glabra (Gg) and two techniques were performed namely; wash and spin technique and density gradient technique. The patients were divided into eight groups according to male infertility factors. The results showed that the mean of sperm concentration in the eight groups by using two activation techniques with free-Gg medium and with 0.1% G. glabra medium, was significantly (P<0.001) lower than that of before activation. Whereas, the percentage of sperm motility and morphologically normal sperm(MNS) after activation by using density gradient technique with medium containing 0.1% Gg was significantly (P<0.001) higher in all groups than that of before activation and after activation by using wash and spin technique. Also, percentage of sperm DNA fragmentation, the number of round cells and sperm agglutination percentage after activation by using density gradient technique with medium containing 0.1% G. glabra were significantly (P<0.001) lower than that of before activation and after activation by using wash and spin technique in all groups. According to the data of the present study, the best results of certain sperm characters were noticed when using a medium containing 0.1% Gg with density gradient technique for diabetic and varicocele men. This data can be utilized to enhance the output of diabetic and varicocele patient through assisted reproductive technologies programs.